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Proteomic analysis of colonic mucosa in a rat model of irritable bowel syndrome.

Proteomics. 2010 May 11;

Authors: Ding Y, Lu B, Chen D, Meng L, Shen Y, Chen S, Lu B

Irritable bowel syndrome (IBS) is one of the most common functional disorders of the gastrointestinal tract. It is characterized by abdominal pain and changes in bowel habits. Various studies have investigated the pathophysiologic processes underlying IBS, but the mechanism remains poorly understood. In the present study, we established an IBS model and identified differentially expressed proteins in colon tissue of IBS rats compared with healthy controls by two-dimensional (2-D) gel electrophoresis, matrix assisted laser desorption/ionization time-of-flight (MALDI-TOF) mass spectrometry, and Western blot analysis. Our results showed that thirteen of the 1396 protein spots on 2-D gel were differently expressed between the IBS and control groups. Ontological analysis of these proteins revealed primary roles in catalytic activity (protein disulfide-isomerase A3 [PDIA3], glyoxalase I, cathepsin S, alpha-enolase), structural support (cytokeratin 8 [CK8]), antioxidant activity (peroxiredoxin-6), protein binding (transgelin, serpin peptidase inhibitor B5), and signal transduction (40S ribosomal protein SA). PDIA3 and CK8 overexpression in IBS were confirmed by Western blot. The findings indicate that multiple proteins are involved in IBS processes that influence intestinal tract immunity, inflammation, and nerve regulation. Our study provides useful candidate genes and proteins for further investigation.

PMID: 20461717 [PubMed - as supplied by publisher]

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